Details: catheter (polyurethane epicutaneo-cave catheter -24g/2 fr- 2 piece2. hamilton syringe 10 ul- 1 piece3. mg/np-40 extraction buffer – 500 ml4. polyethylene glycol- 250 gm viscous liq.5. ammonium acetate – 250gm6. 100% acetone- 500 ml7. sucrose- 500 gm8. methanol- 1000 ml9. phenol- 100 ml10. dab (3,3-diaminobenzidine tetrahydrochloride)- 100 ml11. triton x-100- 100 ml12. polyclonal rabbit anti-cow gfap antibody – 500 ul13. alexa fluor 488 conjugated goat anti-rabbit secondary antibody – 1 mg14. goat serum – 100 ml15. dapi – 25 mg16. h2o2 – 100 ml17. manganese chloride (mncl2) – 10 gm18. peroxidase (hrp)-labeled lectin from griffonia simplicifolia (b s- isolectin b4;catalog # l-5391) – 0.2 mg19. sodium citrate – 5 gm20. sodium azide – 5 gm21. milli q water – 5 litre22. p-phenylenediamine – 5 gm23. amicon 30 kda mwco filter 4 ml sample volume– 1 pack
Sector: Higher Education