Details: transcriptom sequencing along with comprehensive analytics1. whole transcriptome sequencing: whole transcriptome sequencing using illuminasequencing platforms to study differential expression analysis as per experimental design.items specificationsquantity 1rna extractionno. of samples: 18extraction of high-quality total rna from tissue for ngs experimentsfrom silk worm tissue samplesillumina librarypreparation &deep sequencingno. of samples: 18samples processing: rna qc & ribo depletion using appropriate kittotal rna libraries with insert sizes: ~180-200 bpmodule: pe150 module on illumina short read platformdata: 15-20 gb of >q30 data per sample should be generated usingillumina 2x250 pe modulecomprehensivetranscriptomeanalytics raw data quality control & filtering of data should be performed samples wise alignment & expression profiling for all the samples expression normalization & samples correlation analysis shouldbe performed differential expression profiling as per experimen
Sector: Agriculture Misc