Description: 01. Trypsin EDTA Solution 10X 1 (10 ml) Any NA 02. Dulbecco’s Modified Eagle Medium (DMEM), High glucose 20 (500 ml each) Any NA 03. RPMI-1640 1 (500 ML) Any NA 04. Dulbecco’s Modified Eagle Medium / Nutrient Mixture F-12 Ham (DMEM / F12, 1:1 Mixture) 1 (500 ML) Any NA 05. Dimethyl Sulphoxide (DMSO), molecular biology grade 1 (500 ml) Any NA 06. 6.5 mm Transwell with 8.0 ?m pore polycarbonate membrane insert, TC-treated, w/lid, sterile 1 pack Corning CLS3422-48EA 07. Blasticidin S Hydrochloride for cell culture, 98% 1 (10 gm) SRL 92370 08. Manganese (II) Chloride tetrahydrate 1 (500 gm) SRL 75113 09. Agarose, low EEO, for molecular biology 1 (50 gm) Himedia MB002 10. PIPES, molecular biology grade 1 (100 gm) SRL 49149-100 gm 11. Sodium orthovanadate, pure, 45% 1 (10 gm) SRL 86766 12. 2-Mercaptoethanol, pure 1 (100 ml) SRL 69892 13. Bradford Dye Reagent 1 lt (1X) BioRad #5000205 14. N,N-Methylene Bisacrylamide (bis-Acrylamide) pure, 98% 1 (100 gm) SRL 38862 15. Sodium bicarbonate 1 (500 gm) SRL 92957 16. Hematoxylin solution (Harris) for microscopy 125 ml SRL 40362 17. Hydrochlroic Acid 6N Aq solution 1 (1 lt) SRL 17560 18. CUEDC2 antibody 1 Cell Signaling Technology 12294S 19. Estrogen Receptor ? antibody 1 Cell Signaling Technology 8644S 20. APC11 antibody 1 Cell Signaling Technology 14090S 21. KLF4 antibody 1 (20 ul) Abclonal A6640 (20 ul) 22. Androgen receptor antibody 1 (20 ul) Abclonal A2053 (20 ul) 23. EcoRI enzyme 10000 units New EnglabBiolabs/Takara/Thermo Fisher/Promega NA 24. NotI enzyme 10000 units New EnglabBiolabs/Takara/Thermo Fisher/Promega NA 25. XhoI enzyme 5000 units New EnglabBiolabs/Takara/Thermo Fisher/Promega NA 26. BamHI enzyme 10000 units New EnglabBiolabs/Takara/Thermo Fisher/Promega NA 27. BbsI enzyme 300 units New EnglabBiolabs/Takara/Thermo Fisher/Promega NA 28. T4 polynucleotide kinase 500 units New EnglabBiolabs/Takara/Thermo Fisher/Promega NA 29. T4 DNA ligase 20000 units New EnglabBiolabs/Takara/Thermo Fisher/Promega NA 30. Hot Start DNA polymerase 200 units New EnglabBiolabs/Takara/Thermo Fisher/Promega NA 31. SuperScript™ IV First-Strand Synthesis System 18091050 ThermoFisher
Sector: University