Description: Item Category Custom Bid for Services
Details: 1.Isolation of total DNA from leaf endosphere & rhizospheric soil samples under aseptic conditions for microbiome study.Qty12 samples2. Amplification of V3-V4 region (for bacteria) & ITS1 - ITS4 region (for fungi) using isolated total DNA as templates.3.Library preparation & sequencing in an Illumina platform with paired end chemistry to obtain at least 50,000 high quality reads/sample.4. Bioinformatic analysis of reads including removal of poor quality & adapter sequences, merging of pre-processed reads, removal of chimeric sequences, picking of Operational Taxonomic Units (OTUs), clustering & taxonomic classification, rarefaction curve, alpha & beta diversity analyses, core microbiome analysis & microbial co- occurrence pattern analysis,
Sector: Forest and Environment