Details: RNA TRANSCRIPTOME (mRNA) SEQUENCING WITH STANDARD ANALYSIS:Whole Transcriptome sequencing using Illumina Sequencing Platforms to study differentialexpression analysis as per experimental design.Items SpecificationsUnit 1RNA Extraction Extraction of high-quality total RNA for sequencing experimentsfrom total of 4 samples with 5 replicationsIllumina LibraryPreparation & DeepSequencingSamples Processing: RNA QC & Ribo Depletion using appropriatekitTotal RNA Libraries with Insert Sizes: ~180-200 bpModule: PE150 Module on Illumina platform (NovoSeq 6000)Data: 30-35GB/sample with >Q30 data per sample should begenerated, all the samples to be sequenced in a single runComprehensiveTranscriptome Analytics Raw data quality control & filtering of data should beperformed. Contamination removal & Qc Check to beperformed and trimmed. Transcriptome assembly using appropriate tools should bedone Transcriptome Assembly validation with various methodsincluding BUSCO should be performed ORF Predictio
Sector: Agriculture Misc