Details: tender for transcriptome sequencing along with comprehensive analytics1. whole transcriptome sequencing: whole transcriptome sequencing using illuminasequencing platforms to study differential expression analysis as per experimental design.items specificationsunit 1rna extractionextraction of high-quality total rna for sequencing experiments fromtotal of 35 samplesillumina librarypreparation &deep sequencingsamples processing: rna qc & ribo depletion using appropriate kittotal rna libraries with insert sizes: ~180-200 bpmodule: pe150 module on illumina platformdata: 30-35 gb of >q30 data per sample should be generatedcomprehensivetranscriptomeanalytics raw data quality control & filtering of data should be performed transcriptome assembly using hq short read & appropriate toolsshould be done transcriptome assembly validation using busco should beperformed orf prediction, annotation & classification of annotatedtranscriptome comprehensive transcriptome annotation using sequencehomo
Sector: Agriculture Misc